reference strains e coli atcc 35218  (ATCC)

Journal: Annals of Clinical Microbiology and Antimicrobials

Article Title: Rapid screening for antibiotic resistance elements on the RNA transcript, protein and enzymatic activity level

doi: 10.1186/s12941-016-0167-8

Figure Lengend Snippet: Detection of TEM β-lactamase mRNAs and the respective protein in E. coli . a FISH staining of TEM β-lactamase mRNA in E. coli harboring pLitmus38 ( red ) and the ribosomal RNAs by Enterobac ( green ). b Antibody ( green ) and DNA/DAPI ( blue ) staining of the TEM β-lactamases in E. coli harboring pLitmus38 ( left ) or pUC18 plasmids ( right )

Article Snippet: Escherichia coli strain DH5α carrying either the high copy number plasmids pLitmus38 (AMP r ) and pUC18 (AMP r ) or the medium copy number vector pBR328 (AMP r , TET r , CHL r ) with a low plasmid stability were used as well as the E. coli reference strain ATCC 35218, a reference strain producing TEM-1 β-lactamases [ – ].

Techniques: Staining

Journal: Annals of Clinical Microbiology and Antimicrobials

Article Title: Rapid screening for antibiotic resistance elements on the RNA transcript, protein and enzymatic activity level

doi: 10.1186/s12941-016-0167-8

Figure Lengend Snippet: Simultaneous staining of TEM mRNA and TEM protein in E. coli harboring pLitmus38. The upper image shows the merge of the mRNA signal ( red ), the protein level ( green ) and the DNA/DAPI staining ( blue ). The three images below show each fluorescence channel separately

Article Snippet: Escherichia coli strain DH5α carrying either the high copy number plasmids pLitmus38 (AMP r ) and pUC18 (AMP r ) or the medium copy number vector pBR328 (AMP r , TET r , CHL r ) with a low plasmid stability were used as well as the E. coli reference strain ATCC 35218, a reference strain producing TEM-1 β-lactamases [ – ].

Techniques: Staining, Fluorescence

Journal: Annals of Clinical Microbiology and Antimicrobials

Article Title: Rapid screening for antibiotic resistance elements on the RNA transcript, protein and enzymatic activity level

doi: 10.1186/s12941-016-0167-8

Figure Lengend Snippet: Detection of resistance in mixed microbial samples. Left FISH staining of TEM β-lactamase mRNA in resistant E. coli ( red ), ribosomal RNA staining of all Enterobacteriaceae ( green ) and DNA/DAPI staining of all bacteria in the sample. Right Antibody staining of TEM β-lactamases expressing E. coli ( green ) and DNA/DAPI staining of all bacteria in the sample

Article Snippet: Escherichia coli strain DH5α carrying either the high copy number plasmids pLitmus38 (AMP r ) and pUC18 (AMP r ) or the medium copy number vector pBR328 (AMP r , TET r , CHL r ) with a low plasmid stability were used as well as the E. coli reference strain ATCC 35218, a reference strain producing TEM-1 β-lactamases [ – ].

Techniques: Staining, Bacteria, Expressing